Recently I noticed that many of my ASP.NET applications that work with a SQL Server Express database that resides in the App_Data directory stopped working. The culprit error was "Generating user instances in SQL Server is disabled. Use sp_configure 'user instances enabled' to generate user instances.". I suspect that a SQL Server update went out that disables user instances. I have not been able to substantiate this suspicion yet.
SOLUTION:
The solution is to enable user instances with the following command:
sp_configure 'user instances enabled','1';
Disable user instances with the following command:
sp_configure 'user instances enabled','0';
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